Equipment for reconstitution

Before beginning, ensure you have the following equipment available in your research environment:

• Bacteriostatic water (BAC water) — the standard solvent for peptide reconstitution
• Insulin syringes — 1mL with 100 IU markings recommended
• Alcohol swabs for sterilising vial tops
• Refrigerator (2-8°C) for storage after reconstitution
• Freezer (-20°C) for long-term lyophilised storage

Step-by-step reconstitution protocol

• 1
  Allow to reach room temperature Remove the lyophilised vial from cold storage and allow it to equilibrate to room temperature for 15-30 minutes before opening. This reduces thermal stress on the peptide during reconstitution.
• 2
  Calculate your volume Use our Peptide Calculator to determine the exact volume of bacteriostatic water required to achieve your desired research concentration. Record this volume before proceeding.
• 3
  Sterilise both vial tops Wipe the rubber stopper of both the peptide vial and the bacteriostatic water vial with a fresh alcohol swab. Allow to air dry for 10-15 seconds.
• 4
  Draw the bacteriostatic water Using a fresh insulin syringe, draw the calculated volume of bacteriostatic water. Remove any air bubbles by gently tapping and expelling.
• 5
  Inject slowly down the side of the vial Insert the needle through the rubber stopper and angle it so the water runs slowly down the inside wall of the vial — do not inject directly onto the lyophilised powder. This preserves the peptide's molecular structure.
• 6
  Gently swirl — never shake Gently rotate the vial between your fingers until the powder has fully dissolved. The solution should become clear. Do not vortex or shake — this can degrade the peptide through mechanical stress.
• 7
  Inspect before use The reconstituted solution should be clear and free of particulates. If you observe cloudiness, precipitation, or unusual colour, do not use the solution — contact us for guidance.
• 8
  Label and refrigerate immediately Label the vial with the peptide name, concentration, reconstitution date, and your initials. Store immediately at 2-8°C. Do not freeze reconstituted peptide solutions.

Storage conditions by state

Handling best practices

Avoid repeated freeze-thaw cycles — each cycle degrades peptide integrity. If you anticipate multiple draws over time, reconstitute the full vial and draw individual research doses, storing the reconstituted solution at 2-8°C rather than freezing and thawing repeatedly.

Protect from light — UV exposure can degrade peptide bonds. Store vials in their original packaging or wrapped in foil when not in use.

Use sterile technique throughout — contamination of the vial is the primary cause of degradation and failed experiments. Always use a fresh needle for each draw and never reuse syringes.

GHK-Cu note — GHK-Cu solution may have a faint blue-green colour due to the copper chelation. This is normal and does not indicate contamination or degradation.